Samuel O Adeosun

7 Questions 7 Answers 0 Followers

Questions related from Samuel O Adeosun

Converting in-vitro cell culture dose to in-vivo dose in mice - any thoughts?

I am trying to determine the concentration/dose of a drug that was effective on mouse primary neurons at 100-500nanoMolar. I'm planning to inject intracerebroventricularly (ICV), is there a...

23 April 2014 1,785 6 View

Has anyone used the iBind Hands-free Immuno-detection system?

I came across the description of the iBind system from Life technology and I find it interesting. I'm wondering whether someone has used the device so as to know whether it works really well as...

01 March 2014 647 3 View

Induction of Caspase 3 by Staurosporine in mouse primary neurons: Dosing & Timing?

I'm trying to induce caspase activation in primary neurons with staurosporin treatment. Can someone help with what concentration I should start with and duration of treatment to see an easily...

09 January 2014 7,088 11 View

Small Molecules to label Amyloid and Tau for In vivo or Ex Vivo brain Imaging

What kinds of small molecules can be used for fluorescent or luminiscent labelling of Amyloid beta and/or Tau for In-vivo or ex-vivo imaging of the whole brain? We're looking for something that...

27 February 2013 8,537 9 View

Western Blot Protocol for Amyloid beta

Can anyone help with a working protocol for amyloid beta detection in mouse brain by western blot? I've tried using two different antibodies (abcam and CellSignalling) but none of them worked. I...

24 January 2013 9,660 6 View

Which AD hallmarks does Thioflavin S label? Amyloid-β plaques or Neurofibrilary tangles? Or both?

I was trying to get an easier/improved method for Thioflavin S staining and came across different authors some of which said Thioflavin S stains tau-based neurofibrillary tangles/paired helical...

20 December 2012 6,135 17 View

What type(s) of cell lines can be used to study neurogenesis in-vitro?

I'm also looking into double- or triple fluorescence labelling the different cell types. Any reference materials will be appreciated

17 August 2012 7,440 6 View