Sams M A Sadat

3 Questions 3 Answers 0 Followers

Questions related from Sams M A Sadat

What is best way to show the relationship between Caspase 7 induction and cell signalling pathway or other cellular proteins in PTEN knocked-out cell?

Can anyone help: what is best way to prove or show the relationship between Caspase 7 induction and cell signalling pathway or other cellular proteins in PTEN knocked-out colorectal cancer cell...

02 February 2019 8,377 2 View

How can I extract or isolate an antibody that is water soluble?

I have an antibody with other ingredients which is completely water soluble. How I can extract or isolate the antibody? The molecular weight of the antibody is 148 kda.

03 March 2014 8,955 3 View

Can lysis buffer (e.g. RIPA or other types) denature the drug bound protein?

My drug is specific to a nuclear protein. Trying to measure the intra-cellular binding affinity of drug to the target nuclear protein. I have tried to do the lysis of the cells using liquid...

01 January 1970 6,311 2 View