Sameena Alam

6 Questions 9 Answers 0 Followers

Questions related from Sameena Alam

Why no image of Ligated mRNA on the 1% gel with Sybr safe stain?

Hello Fellow researchers ! I am doing RLM 5'RACE to validate mirRNA cleavage on it's target genes using GeneRacer kit (Thermo Fisher Scientific). After when purified mRNA using Dynabeads where i...

01 February 2024 6,210 3 View

Why mRNA isolation is very low through Dynabeads mRNA purification kit 61006 ?

Hello Fellow researchers !!!! I have been trying to isolate mRNA from extracted RNA (Alfalfa Plant leaves) using Dynabeads mRNA purification kit. I have been following the protocol exactly the...

02 January 2024 2,175 8 View

Doubt in qPCR results?

Hello!! Can anyone tell what could be the reason to get completely opposite Cts value in 2 different qPCR with same qPCR components? I was testing a certain primer on different tissues, first...

04 December 2023 562 3 View

Doubts on mRNA isolation using Dynabead purification kit?

My work is on microRNA on alfalfa. I need to purify and isolate mRNA from Alfalfa extracted RNA using Dynabead purification kit. The protocol given was for 75ug of RNA. I don't need that much...

28 November 2023 8,776 1 View

Can anyone explain me difference between Taqman and SYBR green assay?

I am working on miRNA and I am advised to used SYBR green but I have seen people using Taqman, so I am confused. It will really help

12 October 2023 2,259 2 View

How to remove DNA contamination in miRNA isolation?

Hello everyone, I am new in research. I am having problem in removing DNA contamination from RNA isolated through TRizol method. I am working on miRNA overexpression on Alfalfa. Since miRNA size...

04 October 2023 1,985 3 View