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Questions related from Sameena Alam
I am new to CRISPR and we are trying multiplex genome editing in plants for 3 guideRNAs in a vector for a gene. We are followingA CRISPR/Cas9 toolkit for multiplex genome editing in plants...
16 December 2024 2,439 2 View
Hello Fellow researchers ! I am doing RLM 5'RACE to validate mirRNA cleavage on it's target genes using GeneRacer kit (Thermo Fisher Scientific). After when purified mRNA using Dynabeads where i...
01 February 2024 6,284 3 View
Hello Fellow researchers !!!! I have been trying to isolate mRNA from extracted RNA (Alfalfa Plant leaves) using Dynabeads mRNA purification kit. I have been following the protocol exactly the...
02 January 2024 2,272 8 View
Hello!! Can anyone tell what could be the reason to get completely opposite Cts value in 2 different qPCR with same qPCR components? I was testing a certain primer on different tissues, first...
04 December 2023 640 3 View
My work is on microRNA on alfalfa. I need to purify and isolate mRNA from Alfalfa extracted RNA using Dynabead purification kit. The protocol given was for 75ug of RNA. I don't need that much...
28 November 2023 8,834 1 View
I am working on miRNA and I am advised to used SYBR green but I have seen people using Taqman, so I am confused. It will really help
12 October 2023 2,311 2 View
Hello everyone, I am new in research. I am having problem in removing DNA contamination from RNA isolated through TRizol method. I am working on miRNA overexpression on Alfalfa. Since miRNA size...
04 October 2023 2,033 3 View
