Samanvita Ravikanti

3 Questions 1 Answers 0 Followers

Questions related from Samanvita Ravikanti

Caco-2 Passage Number?

Hi Everyone, I am currently doing some experiments with caco-2 cells. I'm looking at barrier integrity and probiotic conditioned treatment. I'm just trying to see what passage number the cells...

19 May 2025 4,828 1 View

MTT yielding very low absorbance values in Caco-2 cells?

Hi everyone, I am trying to do an MTT with caco-2 cells. I am following the same general protocol for an MTT for a 24 well plate. 1. Seed cells 48h 2. Add 100ul of MTT solution 3. Incubate...

25 April 2025 6,847 2 View

Caco-2 cells won't grow in inserts?

Hello, I am working with caco-2 cells. I use DMEM with 10% FBS and NEAA and antibiotics. My cells are growing fine in T75 flasks and 24 well plates. When I try to seed in inserts, they are getting...

14 October 2024 8,638 2 View