Salim Timo Islam

2 Questions 15 Answers 0 Followers

Questions related from Salim Timo Islam

What is the best (non-antibody) reagent to use to covalently (or otherwise) label an exposed His8 tag?

Option 1: I would like to label an exposed His8 tag with a compound that will stay bound to the His8 tag during denaturing SDS-PAGE resolution of protein bands. In this way, non-labeled proteins...

11 November 2012 9,237 10 View

Why does the turbidity of my detergent-solubilized mixture of lipid and membrane protein change depending on the protein:lipid ratio?

Scenario: --> 5 tubes, all with the same amount of dried-down lipid (e.g. 1 mg). --> In the end, each 1 mg of lipid will be solubilized in the same final volume (e.g. 1 mL) --> in the end, each...

11 November 2012 6,558 10 View