5 Questions 8 Answers 0 Followers
Questions related from Sakil Mahmud
I am trying to clone with a pMAL-C2X vector carrying BamHI and SalI sites. I performed several times the ligation with the PCR insert and found several colonies. But seems all are empty vectors....
14 February 2023 3,950 3 View
For ratiomteric analysis, I am currently working with Leica LASX which provides the files of multi-channel in .lif files only. But, I have to use Redox Ratio Analysis Software which can only...
24 June 2019 3,963 0 View
For measuring hormones, I wanted to collect the leaves of directly connencted vascular systems at rosette stage. But, it seems like the plants are already flowering and the leaf orientation is not...
03 December 2018 1,385 3 View
As Jasmonic acid is volatile, so what would be the best way to keep its stable form during treatment in 1/2 MS plates? And I also want to apply Jasmonic acid during seedling stage. So what would...
24 April 2018 3,874 10 View
My protein has 3 isoforms and their product sizes are about 1500 bp and I wanted to clone those isoforms from cDNA through the pBIN19 vector. First I used iProof high fidelity polymerase and from...
15 February 2018 7,079 7 View
