Saiqa Aslam

4 Questions 4 Answers 0 Followers

Questions related from Saiqa Aslam

Is internal control needed for TaqMan Probe-based Real-time PCR Assay?

Hello everyone! I am using TaqMan probe-based Real-time PCR for the detection of typhoidal salmonella. Since I am not concerned about the expression level of gene, I just want to detect if there...

29 January 2024 6,568 2 View

How to calculate DNA copy number/ul if I know the DNA concentration in nanograms/ul?

My gene of interest is 312bp in length while the amplicon size (size of my PCR product) is 212 bp. I have cloned it in to pUC57 plasmid (2710bp) to use it as a positive control in qPCR. so while...

13 December 2023 2,570 1 View

Why am I getting amplification of my genes in negative control in Real-time PCR?

Hi there! I got amplification of my genes in the negative control in Real-time PCR experiment although the Ct values were above 30. I setup my reaction again after making new dilutions of PCR...

24 June 2023 4,242 9 View

Hi there! I run a Real-time qPCR for amplification of my target DNA and I got the Ct value of 4 for one of my DNA sample. What does that mean?

Hi there! I run a Real-time qPCR for amplification of my target DNA and I got the Ct value of 4 for one of my DNA sample. What does that mean? Does it mean that I have too much temple DNA in that...

25 November 2022 1,473 0 View