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Hi there! I got amplification of my genes in the negative control in Real-time PCR experiment although the Ct values were above 30. I setup my reaction again after making new dilutions of PCR...
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Hi there! I run a Real-time qPCR for amplification of my target DNA and I got the Ct value of 4 for one of my DNA sample. What does that mean? Does it mean that I have too much temple DNA in that...
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