Saikiran Kumar Kandukuri

6 Questions 10 Answers 0 Followers

Questions related from Saikiran Kumar Kandukuri

Could anyone share me the articles related for the determination of extinction coefficient by Amino acid analysis and Dry weight method?

Dear All, I am planning to develop a method for(monoclonal antibodies) extinction coeffcient determination inhouse using amino acid analysis and dry weight method. I was looking for the right...

16 April 2018 7,583 3 View

Can anyone share me the protocol for extrinsic fluorescence analysis using CCVJ dye?

Hi all, I have been using CCVJ dye for extrinsic fluorescence analysis to investigate tertiary structure of protein. The dye has been dissolved in DMSO which is of 20mM and further diluted to 5uM...

23 September 2016 5,589 3 View

Multiple bands in Non-Reducing SDS PAGE. Can anyone suggest me the probable reasons?

Hi All, I have performed Non-Reducing SDS-PAGE for a pure antibody where I have observed multiple bands instead of a single intact band. Kindly suggest the probable reasons.

13 September 2016 7,455 10 View

Additional Bands in Reducing SDS PAGE. Can anyone suggest the appropriate reasons behind this?

Hi All, I have been performing Reducing SDS PAGE for pure antibody where I could find additional bands apart from heavy and light chain with molecular weight around 75 to 100kDa and at 100 to...

13 September 2016 2,683 5 View

How can we determine tertiary structure of protein using CCVJ(9-([E]-2-Carboxy-2-cyanovinyl)julolidine) dye for extrinsic fluorescence?

Hi all, I would like to use CCVJ dye for investigating the tertiary structure of protein using extrinsic fluorescence. I have the dye with me and bit confused into which the dye need to be...

06 January 2016 1,884 3 View

Can anyone share the sample preparation of CCVJ(9-(2-Carboxy-2-cyanovinyl) julolidine) dye for mabs?

Hi all, Anyone who has experience in performing fluorescence assays to investigate the tertiary structure of protein

02 January 2016 6,570 1 View