Saikal Shamkeeva

3 Questions 2 Answers 0 Followers

Questions related from Saikal Shamkeeva

Has anyone done exosome injections ip in mice? how did you calculate the concentration for injections?

I have seen several articles that are using protein concentration calculations (e.g. injecting 200ug of EVs), however, my isolation method is very specific, thus protein level is low. I used an...

07 August 2023 8,978 0 View

How well human monocytes isolated from PBMC growing and propagating in cell culture?

I am planning to isolate human monocytes from buffy coat and wondering how well isolated monocytes can grow in cell culture? if I need more than 4.5 million cells per flask, how much blood is...

25 October 2021 5,180 3 View

What can possibly cause only negative droplet generation in ddPCR reaction?

Dear all, I have started experiment with ddPCR using previously established protocol on methylation analysis. However, my first run did not work well. I could only see a negative droplets in all...

06 January 2021 8,671 2 View