Said Nawab

4 Questions 2 Answers 0 Followers

Questions related from Said Nawab

Hello everyone, Does anyone familiar with golden gate assembly for plasmid construction?

I wanna to construct plasmid using golden gate assembly, I haven,t used it before. I have three insert (each lenght is about 180 bp) and one plasmid backbone (Lenghth 3.5kb). Total four...

02 February 2019 2,238 7 View

Does any one know the endnote style format (Biotechnology for biofuels)?

Dear All, I did not found the endnote style of the journal (Biotechnology for biofuels) on the home page. Does any one have the information, Please share with me. Thanks

10 October 2018 3,672 3 View

Could anyone let me know efficient method of doing colony PCR in Corynebacterium glutamicum?

I am following same protocol as in E. coli. Mix a colony in 20 ul of distilled water, aliquot 2 ul in PCR tube, heat at 95°C for 10 min and follow regular PCR reaction and cycle but it does not...

08 August 2018 935 3 View

Can methylated plasmid transform haloferax mediterranei strains (Haloarcheae)?

I am working on Haloferax mediterranei strains. I wanna to transform the Haloferax mediterranei with plasmid constructed in Top 10 E. coli strain. I tried so many times but failed to transform the...

04 April 2018 3,876 3 View