Restriction digestion fails to release cloned insert from pET101/D-TOPO vector - can anyone help?

I have done a TOPO cloning of a 450 bp fragment (amplified with Pfu Pol using forward primer designed with 5'CACC(ATG) and reverse primers having no 5' CACC) into pET101/D-TOPO vector in 1:1 and...

12 December 2013 3,716 7 View