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Questions related from Sai Shilpa Kommaraju
Hello I am trying to do retrograde tracing from liver to spinal cord using AAV-retro-EF1a-DIO-deltaG-WPRE-hGH (AAV helper virus) along with G-deleted Rabies-eGFP. These both can be injected...
29 March 2021 3,795 0 View
I have been using mouse leptin ELISA kit (Catalog number (90030) from crystal chem. I have been doing manual washing between my steps as I didn't have automatic washer instrument. Does this cause...
13 August 2020 4,481 4 View
Hello I have been trying to express and purify two truncated his-tag proteins(45KDa and 65KDa size without his-tag) using baculovirus expression system. I was however able to express the proteins...
22 July 2017 7,555 7 View
I have prepared samples in SDS loading buffer to load the gels 5 days back. Can I still use the same samples which are already prepared to repeat the western blot because I don't have enough...
31 March 2015 8,756 6 View
I have been using DH10Bac competent cells for transformation of my recombinant DNA, I want to make those at lab instead of purchasing commercially every time. Can I use the normal procedure using...
16 March 2015 6,088 6 View
