Saheli Halder

3 Questions 9 Answers 0 Followers

Questions related from Saheli Halder

Has anyone experienced missing nucleotide sequence even without any perturbation?

I have experienced a strange thing while my recent cloning. After I had sequenced my clone i noticed that a part of the nucleotide sequence got missing from the plasmid backbone. I have not done...

09 September 2019 5,570 2 View

Can Bluo-Gal be used instead of X-Gal for Topo-TA cloning?

I was wondering if Bluo-Gal can be used instead of X-Gal for blue white screening for Topo-TA cloning? If yes, then what is the concentration to be used for spreading on the LB agar plate? We do...

07 July 2019 3,830 2 View

Protein extraction from insect cells?

I am trying to extract a soluble protein from Insect cells. After infection I see cell swelling. I harvest the cells at about 45-50% cell viability (3-4 days post infection). I see cell swelling....

04 April 2019 3,339 4 View