Phusion HF DNA polymerase fails to amplify whereas Taq DNA polymerase succeeds. What could be the reason for this?

Hello, I am trying to PCR amplify a 4kb gene sequence from a vector. The primers used are bit lengthy (>40bp). I have tried 20 different annealing temperatures from 50 to 70 deg C, I have even...

19 July 2019 9,869 5 View