Sagar Kittur

2 Questions 3 Answers 0 Followers

Questions related from Sagar Kittur

Phusion HF DNA polymerase fails to amplify whereas Taq DNA polymerase succeeds. What could be the reason for this?

Hello, I am trying to PCR amplify a 4kb gene sequence from a vector. The primers used are bit lengthy (>40bp). I have tried 20 different annealing temperatures from 50 to 70 deg C, I have even...

19 July 2019 9,919 5 View

How efficient is the assay method of estimation of Hyaluronic acid by treating it with SDS followed by colorimetric estimation using carbzole reagent?

Hello, I am trying to verify the efficacy of an assay method to quantify standard concentrations of Hyaluronic acid (HA). I follow the protocol which is mentioned below. It is a colorimetric...

15 October 2016 7,905 8 View