Sadie Grant

6 Questions 2 Answers 0 Followers

Questions related from Sadie Grant

How to quantify protein/peptide samples that contain pigment?

I am working with microbial protein samples extracted from mouse gut microbiome samples. These mice were fed 13C algal protein (Sigma 642878) which is pigmented so the samples contain pigment,...

last month 7,604 3 View

How can I mix a powdered prebiotic into pelleted mouse chow?

Hello, I want to administer a powdered prebiotic into pelleted mouse chow, and I wanted to see if anyone has a protocol or advice for that. I've read papers where researchers smash powdered inulin...

30 April 2023 9,075 2 View

What is the best protocol for adapter ligation?

Hi all, I've been attempting to ligate illumina adapters to a PCR amplified dsDNA fragment (sticky ends, 4bp overhang) but on all of my gels I can see the adapter and the insert separately, not...

10 February 2022 486 2 View

How to avoid extra bands while amplifying PCR products?

I am working on a method where I need to amplify a short (150bp) sequence, and then I want to amplify that PCR product to increase concentration. The goal is to obtain a clear 150bp band after the...

28 December 2021 1,000 7 View

Why are my ligations failing?

I'm trying to ligate two adapters to a linear insert of DNA. The adapters are 59 and 69 bp long, and the insert is ~200bp. It's approximate because there are many inserts, they are the result of a...

05 November 2021 736 1 View

Can DNA self ligate after restriction digestion/ligation?

Goal: I'm trying to create a concatemer of multiple short DNA fragments. Background: I PCR amplified many sequences (112bp), each with two BsaI cut sites. There is a fragment within each sequence...

12 July 2021 1,833 3 View