Sadie Grant

5 Questions 2 Answers 0 Followers

Questions related from Sadie Grant

How can I mix a powdered prebiotic into pelleted mouse chow?

Hello, I want to administer a powdered prebiotic into pelleted mouse chow, and I wanted to see if anyone has a protocol or advice for that. I've read papers where researchers smash powdered inulin...

30 April 2023 9,066 2 View

What is the best protocol for adapter ligation?

Hi all, I've been attempting to ligate illumina adapters to a PCR amplified dsDNA fragment (sticky ends, 4bp overhang) but on all of my gels I can see the adapter and the insert separately, not...

10 February 2022 471 2 View

How to avoid extra bands while amplifying PCR products?

I am working on a method where I need to amplify a short (150bp) sequence, and then I want to amplify that PCR product to increase concentration. The goal is to obtain a clear 150bp band after the...

28 December 2021 987 7 View

Why are my ligations failing?

I'm trying to ligate two adapters to a linear insert of DNA. The adapters are 59 and 69 bp long, and the insert is ~200bp. It's approximate because there are many inserts, they are the result of a...

05 November 2021 730 1 View

Can DNA self ligate after restriction digestion/ligation?

Goal: I'm trying to create a concatemer of multiple short DNA fragments. Background: I PCR amplified many sequences (112bp), each with two BsaI cut sites. There is a fragment within each sequence...

12 July 2021 1,820 3 View