8 Questions 22 Answers 0 Followers
Questions related from Saba Tabasum
I would like to know the exact protocol to induce chemical hypoxia in A549 cells. There are some variations that I have noticed in the method in various papers which are confusing. Do we starve...
06 June 2016 5,403 3 View
I am currently doing invasion assay. I have fixed the matrigel coated inserts after 20 h of incubation with H1299 cells in 2% PFA and stained with 0.5% crystal violet. I have air dried them...
03 March 2016 2,111 6 View
I am currently doing a cell adhesion assay with gelatin coating. Can anyone suggest to me a detailed protocol. I have made 1% gelatin by dissolving 1g gelatin in double distilled water (insoluble...
07 July 2014 8,630 4 View
I work with drugs with anticancer activity, currently I am culturing A549 cells. Would it be ok for me to use ciprofloxacin as an antibiotic as it has chemotherapeutic activity? What should be the...
06 June 2014 3,595 4 View
forward primer-5'-TTC GGG GTG TAG CGG TCG TC-3' reverse primer-5'-GCC CCA ATA CTA AAT CAG GAC G-3' These are the primers for the methylated allele of the gene GSTP1 taken from 'Prostate cancer...
06 June 2014 5,742 6 View
I am trying to induce EMT in A549 cells by using TGF-beta as ligand. Currently, I am standardizing and it involves 24 hours of starvation and 48 hours of incubation with the ligand however by the...
03 March 2014 6,065 2 View
I am doing pharmacokinetic/bioavailability study of a drug in plasma samples by HPLC for the first time. Can somebody tell me the sample preparation of plasma for HPLC? Do I have to deproteinized...
12 December 2013 1,562 7 View
I am trying to induce EMT in A549 cells by using TGF-beta ligand (sigma,T7039), I starve my cells for 24 hours in SFM and then add TGF-beta (5ng/ml) in SFM only and after 24 hours of this...
11 November 2013 2,604 16 View
