Saadlee Shehreen

5 Questions 4 Answers 0 Followers

Questions related from Saadlee Shehreen

Is overexpression of RFP toxic for E.coli BL21?

I am trying to transform a pre-made plasmid in the E.coli BL21. The plasmid has my insert (potential promoter sequence) upstream of the RFP protein. Usually, all my positive clones are pink in...

23 September 2021 2,082 4 View

Troubleshooting electroporation for Pectobacterium?

Hi, I am trying to transform 10 Kbp plasmid vector ( my gene of interest is around 5 kbp) in the Pectobacterium. Usually, we can't make pectobacterium chemical competent, so we make them...

27 June 2019 5,730 1 View

Why there is no insert band in the gel?

Hi, I want to insert six piece of gene in the pBAD30 vector. Those genes were previously cloned into pHRED30T vector ( between NcoI and HindIII restriction sites ). For this work, I chose...

07 June 2019 785 1 View

Is there any available written script to obtain information on Organism name,SRA and Assembly from NCBI?

Hi, I am working on 200 different organisms from NCBI. I have Accession id ( e.g. NZ_KQ956078.1) of those organisms but need the corresponding information on SRA, Assembly number, Organism name...

30 October 2018 4,776 3 View

Why get two PCR bands (above 5kb) of the vector (Plasmid) after cloning?

Hi, I did transformation and found some colonies in the ampicillin plate having Uncut vector and competent cells . And also in the plate with Insert+vector and competent cells. Then, I picked 5...

01 August 2018 1,165 4 View