I am trying to sequence presumptive lactic acid bacteria and was getting answers before but now I am stuck with a primer dimer only.

The primers that I have used are 1)Forward- 16SF, 5 -AGAGTTTGATCCTGGCTCAG-3; Reverse- 16SR, 5 -CTACGGCTACCTTGTTACGA-3 from Scarpellini et al,2002 2)27F (5′-AGAGTTTGATCCTGGCTCAG-3′) and 1492R...

02 February 2013 3,252 1 View