S. Saif Hasan

2 Questions 1 Answers 0 Followers

Questions related from S. Saif Hasan

Can pSUMO sequence be sub-cloned and expressed in mammalian cells?

Hello, I am trying to express a single domain soluble protein with an N-terminal SUMO tag in E coli. As an alternative, I would like to express this fusion protein in mammalian cells. This will...

11 January 2022 1,245 0 View

Signal sequence needed for membrane protein production in S2 cells?

Hello, I am new to using Drosophila S2 cells for protein production. I would like to over-express a membrane protein gene (12 TMH predicted with intra-cellular N and C-terminii) in S2 cells.The...

08 March 2018 3,450 1 View