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Our lab currently uses fiberboard 100-space freezer boxes in metal racks to store our antibodies. The fiberboard gets worn out and the racks are clumsy (they are the sliding drawer type). So, I'm...
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I coat my wells with antigen overnight at 4°C, wash with PBS+0.05% Tween20, block for 1 hour at RT with SuperBlock, and then add either serum from mice with antibodies against the coating antigen...
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