Ryan Pekson

3 Questions 5 Answers 0 Followers

Questions related from Ryan Pekson

How can I perform a viability test for tissue explants?

I'm isolating tissue sections and growing them in T25s and treat them. Afterwhich, i collect supernatants for analysis of released proteins. How can I check the viability of my tissues, like you...

22 December 2014 2,017 3 View

Standardizing supernatant?

I'm collecting supernatant as samples and measuring certain proteins released by cells/tissues after treatment. I would appreciate suggestions on how to standardize supernatant in running Westerns...

12 February 2014 8,688 11 View

Nitric oxide (NO) measurement - fluctuating data?

I'm currently doing Griess assay for measuring NO levels in cell supernatant for which I use the Promega kit. After collecting and centrifuging the supernatant I run the griess assay immediately....

14 August 2013 9,285 1 View