Ryan Coleman

5 Questions 5 Answers 0 Followers

Questions related from Ryan Coleman

How can I quantify ChIP qPCR when IgG control is negative?

I have hit a strange roadblock with my experiments. I am doing a ChIP experiment with an HA-tagged protein in H9c2 cells. I used IgG as an isotype control for my antibody, and after isolating DNA...

01 October 2015 8,064 4 View

Is there a way to verify ChIP when there are no known targets?

I've been working on trying to get a ChIP assay to work on a protein that isn't known to be a transcription factor, but that our lab suspects acts as one in a certain disease state. My project is...

22 September 2015 6,526 14 View

How can I fix a low protein yield in H9c2 cells?

I am starting to work with H9c2 cells. I will be attempting to transfect them with a tagged protein I want to study. I tried a pilot transfection, but when I harvested the cells in about 100 uL of...

30 August 2015 671 2 View

What are a few ways of troubleshooting CHIP?

I'm a first year graduate student and for my project I am attempting to do a chromatin immunoprecipitation, but am having some trouble. I'm using primary neonatal rat ventricular myocytes infected...

23 March 2015 831 1 View

How do I troubleshoot Nystatin partitioning in 80%POPC/20% Cholesterol Liposomes (MLV)?

I've been trying to develop my technique as a graduate student and am attempting to partition the antifungal drug Nystatin into a liposome preparation consisting of 80% POPC/ 20% cholesterol. I've...

11 December 2014 925 3 View