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Questions related from Ryan Coleman
I am having a mouse generated that is supposed to over-express a peptide in a cardiac-restricted manner, using a cardiac-specific alpha MHC promoter. The construct contains the promoter and...
21 April 2016 4,611 5 View
I have hit a strange roadblock with my experiments. I am doing a ChIP experiment with an HA-tagged protein in H9c2 cells. I used IgG as an isotype control for my antibody, and after isolating DNA...
01 October 2015 8,099 4 View
I've been working on trying to get a ChIP assay to work on a protein that isn't known to be a transcription factor, but that our lab suspects acts as one in a certain disease state. My project is...
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I am working with regulator of calcineurin, and need to design primers for qPCR of isoform four. I can find papers published about the isoform, but cannot find a sequence specific to this isoform...
17 September 2015 5,354 0 View
I am starting to work with H9c2 cells. I will be attempting to transfect them with a tagged protein I want to study. I tried a pilot transfection, but when I harvested the cells in about 100 uL of...
30 August 2015 699 2 View
I'm a first year graduate student and for my project I am attempting to do a chromatin immunoprecipitation, but am having some trouble. I'm using primary neonatal rat ventricular myocytes infected...
23 March 2015 866 1 View
I've been trying to develop my technique as a graduate student and am attempting to partition the antifungal drug Nystatin into a liposome preparation consisting of 80% POPC/ 20% cholesterol. I've...
11 December 2014 962 3 View
