5 Questions 8 Answers 0 Followers
Questions related from Ruwini Cooray
I am trying to analyse gene expression after drug treatment in PD cell lines and I came across the "ATCC-DYS0530" PD specific cell line. I was wondering has anyone used this cell line before, and...
29 April 2020 2,389 0 View
Hello I am planning to use human iPSC derived neural stem cells to differentiate and study Alzheimer's disease. For the immunofluorescence assay can I use Tyrosine Hydroxylase? or is it suitable...
19 May 2019 223 2 View
Hi, Can someone suggest me a method of fixing cultured neurons for fluorescent staining and long term storage? Would I not need to go for paraffin fixing since they are cultured cells?
21 November 2018 9,331 3 View
Does anyone know, why would the amplification of CHD gene from female Chicken blood DNA gives only one band at 280-290bp in 3% agarose gel instead of two bands at 281bp and 310bp? I have attempted...
11 July 2017 5,442 6 View
I have been trying to express a kinase recombinant protein in BL21 DE3 and C43 but couldn't get any expression. I used 2L LB culture and 0.5mM IPTG induction. I have even tried with colonies from...
30 July 2016 3,451 2 View
