Ruwini Cooray

4 Questions 8 Answers 0 Followers

Questions related from Ruwini Cooray

Can I use TH for IF with Neural Stem cells differentiated to Alzheimer's Disease?

Hello I am planning to use human iPSC derived neural stem cells to differentiate and study Alzheimer's disease. For the immunofluorescence assay can I use Tyrosine Hydroxylase? or is it suitable...

19 May 2019 189 2 View

Method for fixing cultured neurons?

Hi, Can someone suggest me a method of fixing cultured neurons for fluorescent staining and long term storage? Would I not need to go for paraffin fixing since they are cultured cells?

21 November 2018 9,286 3 View

Chicken CHD gene amplification showed a single band?

Does anyone know, why would the amplification of CHD gene from female Chicken blood DNA gives only one band at 280-290bp in 3% agarose gel instead of two bands at 281bp and 310bp? I have attempted...

11 July 2017 5,409 6 View

Can anyone help me with problem with kinase expression in BL21?

I have been trying to express a kinase recombinant protein in BL21 DE3 and C43 but couldn't get any expression. I used 2L LB culture and 0.5mM IPTG induction. I have even tried with colonies from...

30 July 2016 3,420 2 View