Ruth Purcell

2 Questions 3 Answers 0 Followers

Questions related from Ruth Purcell

How do I transfer a very basic protein to a nitrocellulose membrane?

I need to transfer two very basic proteins (pI of 11.4 and 11.04) and would like to know what transfer buffer is most suitable for these proteins when run on a denaturing gel. CAPS buffer has the...

27 November 2020 7,728 3 View

Does this gel show miniprep plasmids of decent quality?

I performed a miniprep of a plasmid containing an insert of ~1000 bp (total size of construct 7712 bp) and loaded ~650 ng of this miniprep DNA into each well of the below gel. I am worried that...

07 November 2020 368 6 View