Rutendo Kupara

5 Questions 7 Answers 0 Followers

Questions related from Rutendo Kupara

Error: “Cannot enlarge by more than 255 pixels” ?

Using the Angiogenesis analyzer plugin/macro for Image J. I have resorted to converting the image to a binary one and then analyzing the binary tree. (Analyzing HUVEC Fluo was creating features...

03 March 2018 1,153 0 View

How to add or eliminate meshes/junctions/etc. with the angiogenesis analyzer (ImageJ)?

From the Image J Forum (facing similar problem); Hello everyone, I’ve got a problem analyzing my angiogenesis assay with ImageJ. I’m using the Angiogenesis Analyzer from Gilles Carpentier. The...

03 March 2018 6,564 3 View

How can I extract eNOS from rat cardiac tissue?

I need to extract eNOS from rat heart tissue and then assay for its activity, my question is is that a possibility or will I extract both eNOS and iNOS? How can I distinguish between the two, if i...

06 June 2014 8,590 1 View

Griess reagent is used to measure NO released by cells. How can I use this to assess the function of endothelial nitric oxide synthase (eNOS)?

The common method for assessing eNOS function requires addition of radioactive L-arginine, however, I can not use this method, are there other methods available that I can use. Can I perhaps...

05 May 2014 5,965 3 View

Can someone help me understand the purpose of the Z-FHL/HHL ratio when carrying out ACE assays?

After carrying out ACE assays using HHL as a substrate, I wasn't getting good results and I started reading and found that most people use both substrates (Z-FHL & HHL) to carry out the ACE assay...

05 May 2014 3,810 0 View