Rutendo Kupara

3 Questions 4 Answers 0 Followers

Questions related from Rutendo Kupara

What is the best homogenizing buffer for rat heart?

I am looking for the best homogenization buffer particularly for us to evaluate enzyme activity and different enzymes.

02 July 2014 6,902 1 View

How can I extract eNOS from rat cardiac tissue?

I need to extract eNOS from rat heart tissue and then assay for its activity, my question is is that a possibility or will I extract both eNOS and iNOS? How can I distinguish between the two, if i...

19 June 2014 1,974 1 View

Griess reagent is used to measure NO released by cells. How can I use this to assess the function of endothelial nitric oxide synthase (eNOS)?

The common method for assessing eNOS function requires addition of radioactive L-arginine, however, I can not use this method, are there other methods available that I can use. Can I perhaps...

16 May 2014 9,364 2 View