RunQing Yang

5 Questions 12 Answers 0 Followers

Questions related from RunQing Yang

Did anyone have used western to detect CD3ζ chain phosphorylation?

I try very hard to detect CD3ζ chain phosphorylation, but until now, I still can‘t get anything. Here is my TCR stimulation protocol: 1. I resuspend the thymocytes at 1x 107 cells/mL in...

09 September 2016 1,422 3 View

Does anyone know how to induce T cell anergy in vivo?

I give high does(500ug, i.v.) ova peptide to induce OT II mice in vivo, but this method doesn't work. And I still don't know why. I would be very grateful it anyone could tell me how to do this...

01 January 2015 420 5 View

Does anyone know how to do intrathymic transfer?

I want to adoptive transfer some cells into recipients' thymus. But I have no experience on that. Can anyone who has some experience on this method tell me how to do this experiment?

01 January 2015 5,986 2 View

Why do myriad atoms in a fluid fluctuate in their movement, and we can say that in small intervals of time these fluctuations would be unsymmetrical?

Although we can see the result from Brownian motion that small particles in the fluid actually have random movement, which means these fluctuations are unsymmetrical, I still don't know why in...

10 October 2014 6,878 2 View

Does anyone know why the miltenyi magnetic column always plugs when I use it to negative select naive CD4+ T cell?

I have filtered the sample several times before I load it into the column.

08 August 2014 9,733 16 View