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Questions related from Rui Wu
I am basically working with self-assembled VLPs. I am not sure if His tag in my protein is not exposed (I can detect in reducing western blot by his tag antibody) or other technical issues, and...
16 September 2024 7,607 3 View
Due to the dysfunction of cold room of 4 C, I basically created the ice-water bath for my cell lysate dialysis (should be at 4 C in cold room) in dialysis buffer. I periodically check the...
21 March 2022 9,458 0 View
I am trying to using Ni-NTA beads to do small-scale protein sample purifications (from cell-free reactions); however, majority of proteins still bind with sticky bead solutions after running...
01 January 1970 5,613 4 View
Hi all, I am doing cell free protein reactions with His-tag (50 uL), and trying to purify it using Ni beads method (for small scale). After I finish purification and run western blot, there is no...
01 January 1970 7,919 5 View
Hi all, At the beginning, I was using native His-tag purification beads to purify my proteins; however, it still has proteins binding on bears, poorly eluted but can still see faint bands on...
01 January 1970 2,914 3 View
Hi all, I want to isolate and quantify mRNA by qPCR in cell-free protein synthesis as baseline for the comparison of uncoupled cell free reactions. Does anyone know any straightforward way to...
01 January 1970 4,896 3 View
