7 Questions 12 Answers 0 Followers
Questions related from Rudrakshi Sharma
Hi. I have done the yeast transformation for the gene integration using selectable marker hygromycin. I got the colonies within 2-3 days. The colonies are healthy and no contamination is there...
11 December 2023 5,587 5 View
Hello. I am currently tagging a gene of my interest using the auxotrophic marker. The tagging primers which I am using for the process have a long stretch of A's (27) in their sequence due to...
28 June 2023 5,810 2 View
I am working on a vector for gene integration but the information on that vector is given in bits and pieces. I want to construct the complete vector. Can anyone suggest me some software where I...
12 June 2023 764 1 View
Hello people. I have a question. Can I amplify the gene with a dominant maker if my gene already has an auxotrophic marker (already made strain) next to it? Will that auxotrophic marker cause...
06 June 2023 5,994 5 View
Hello everyone. I have a question related to gene integration in the genome of Saccharomyces cerevisiae using a plasmid. Can anyone help me with the gene integration procedure in yeast using the...
11 May 2023 1,645 6 View
As the size of the protein will be larger, more difficult it will be cloned. Can u guys suggest a good way to get the desired mutations in my tagged gene ? Waiting for the answer.
20 October 2021 3,763 3 View
Here are the things : 4kb tagged DNA Extension time provided is of 4 minutes DNA concentration is about 1995.6ng/ul I used 1:10 and 1: 100 dilutions as well but still not getting the...
18 July 2021 4,026 8 View
