Rudrakshi Sharma

6 Questions 12 Answers 0 Followers

Questions related from Rudrakshi Sharma

Regarding yeast transformation Colonies?

Hi. I have done the yeast transformation for the gene integration using selectable marker hygromycin. I got the colonies within 2-3 days. The colonies are healthy and no contamination is there...

11 December 2023 5,575 5 View

Tagging of gene in yeast ?

Hello. I am currently tagging a gene of my interest using the auxotrophic marker. The tagging primers which I am using for the process have a long stretch of A's (27) in their sequence due to...

28 June 2023 5,807 2 View

Gene Amplification with a dominant marker?

Hello people. I have a question. Can I amplify the gene with a dominant maker if my gene already has an auxotrophic marker (already made strain) next to it? Will that auxotrophic marker cause...

06 June 2023 5,986 5 View

How to do Gene Integration In Yeast ?

Hello everyone. I have a question related to gene integration in the genome of Saccharomyces cerevisiae using a plasmid. Can anyone help me with the gene integration procedure in yeast using the...

11 May 2023 1,642 6 View

How can i incorporate site directed mutation in my already tagged gene as the size of the tagged gene will be bigger and difficult to clone ?

As the size of the protein will be larger, more difficult it will be cloned. Can u guys suggest a good way to get the desired mutations in my tagged gene ? Waiting for the answer.

20 October 2021 3,761 3 View

I am working with 4kb DNA which is tagged . I am trying to do PCR using Phu polymerase. Not able to get the band . What to do ?

Here are the things : 4kb tagged DNA Extension time provided is of 4 minutes DNA concentration is about 1995.6ng/ul I used 1:10 and 1: 100 dilutions as well but still not getting the...

18 July 2021 4,023 8 View