Roxane Bories

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Questions related from Roxane Bories

Is it possible to isolate PBMC with Ficoll from frozen blood ?

I tried to isolate PBMC from frozen blood, but nothing happened. Do you know why? Is it because all the cells exploded?

05 February 2025 9,186 1 View

Why my buffy coat stay on top of the Ficoll after 30min of centrifugation ?

Hi, I need to isolate PBMCs from the buffy coat (2 ml). I first diluted my 2 ml of buffy coat with 4 ml of PBS (without Ca/Mg). Then I poured it over 3mL of Ficoll (being very careful not to...

04 February 2025 4,104 2 View