Rowshan Ara Islam

3 Questions 5 Answers 0 Followers

Questions related from Rowshan Ara Islam

Why is my fission yeast aggregating at stationary?

Hi all! My fission yeast strains (wild type and mutants) are growing well in YES. But in minimal media (EMM2, with ammonium chloride), they are aggregating upon reaching the stationary phase. I...

03 May 2023 151 0 View

What is the best media for 4T1 cell culture?

We are currently using DMEM for 4T1. But ATCC protocol mentioned RPMI for 4T1. Should we switch to RPMI or DMEM is good as well?

04 August 2017 5,612 4 View

How confluent my cells should be after 24H of seeding? Mine are getting 70-80% confluent and SD is high even in control wells.Can anybody help?

1.I am using MCF7 and 4T1 cell lines 2. Seeding at 50,000/well concentration in 24 well plate 3. variation among triplicates are high. 4. Sometimes my results differ among control wells, which...

07 March 2017 5,439 7 View