Ross A Cordiner

3 Questions 4 Answers 0 Followers

Questions related from Ross A Cordiner

Protocol for chromatin associated RNA isolation from frozen cells?

Does anyone know of a protocol which I can isolate chromatin associated RNAs from frozen cells. Essentially I am looking to monitor transcriptional output of RNA polymerases during a time-course...

10 October 2018 1,970 2 View

Does anyone have experience with mRNA coverage with Ribo depletion in RNA-seq?

I am starting a new RNA-seq based project. I will be looking at both coding, and non coding RNAs. To enable me to look at both of these RNA species simultaneously, I will be ribo depleting the...

03 March 2014 7,985 0 View

Cross reactivity of RNase with RNA radiolabelling?

I am carrying out the iCLIP protocol and I am having problems with a lot of background in my high RNase control. Instead of seeing a sharp band where at the correct molecular weight of my protein,...

10 October 2013 615 1 View