What is the maximum distance between P5 and read #1 in an Illumina HiSeq amplicon?

I have a minigene library which is amplified with P5/P7 sites that looks like this [P7/barcode][constant region][ variable region ][18nt constant region][SP1][P5] (436nt total) We sequenced this...

05 July 2016 8,985 1 View