Rohit Singh

3 Questions 5 Answers 0 Followers

Questions related from Rohit Singh

What is the site of ribosome binding in pET21+ plasmid?

Hi All, I have a protein sequence cloned in pET21+ vector. Below is the part of the sequence starting from T7 promoter to the ATG (of my...

30 November 2021 4,914 3 View

Determine Kd using tryptophan fluorescence ?

Hello Everyone, I was calculating kd of a 10 aa long peptide with one of my FL protein (MW 25kda, contaning 4 Tryptophane resudes) , It has two domains; one of the domain makes a dimer and other...

17 September 2021 8,940 0 View

Determine Kd using tryptophan fluorescence

Hello Everyone, I was calculating kd of a 10 aa long peptide with one of my FL protein (MW 25kda, contaning 4 Tryptophane resudes) , It has two domains; one of the domain makes a dimer and other...

01 January 1970 7,380 6 View