Rohini Sree

4 Questions 10 Answers 0 Followers

Questions related from Rohini Sree

Problem in quantifying western blot by densitometry

Two bands in the attached blot are very close to each other, and I am unable to quantify separately. Is it fine to quantify together and take an average of that? The bands are not non specific ....

19 June 2013 9,916 15 View

Low TEER readings

In cacao2 cells grown on transwell inserts for around 21-30 days. When seen under the microscope the transwell looks completely confluent but the TEER reading shows it was much less, by around...

11 June 2012 203 8 View

Why are my cells coming out of trans well inserts?

I'm using a colon cancer cell line, after loading in to the transwell, within a week it looks confluent under the microscope but it shows TER only around 200 ohms. If the inserts are left for...

19 April 2012 478 6 View

What is the importance of TER (trans epithelial resistance) and how to measure it?

I'm using the millipore TEER electrode to measure the resistance of colon cancer cell line. I have been unable to get a stable reading, most of the time it shows fluctuations. Although the...

18 April 2012 3,464 10 View