Roger Bradley Dodd

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Questions related from Roger Bradley Dodd

Can NanoDSF be applied to membrane proteins embedded in liposomes or nanodiscs?

Dear all, I would be interested to hear if anyone has applied NanoDSF (as performed by the Prometheus instrument from Nanotemper) to study the unfolding of membrane proteins reconstituted into...

16 November 2015 7,801 1 View

Any advice on fluorescently labelling an oligosaccharide indirectly using dye labelled streptavidin for microscale thermophoresis?

Hi all, I am considering using microscale thermophoresis to characterise the binding of oligosaccharides (700 Da) to a protein (~ 30 kDa). I realise in this case I could label the protein, but I...

14 July 2015 3,261 8 View

Non-specific binding of protein to streptavidin during Octet experiment - any thoughts?

I have been performing some experiments using the ForteBio Octet Red96 system and streptavidin-coated BioSensors. The basic set up of my experiment is to immobilise a biotinylated peptide (4 kDa)...

21 February 2014 7,126 21 View