Roberto Braz Pontes

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Questions related from Roberto Braz Pontes

Has anyone successfully attached freshly isolated endothelial cells from mesenteric arteries to glass-bottom dishes or coverslips?

I'm trying to culture primary endothelial cells after isolation, but I'm struggling with adhesion. I've tried coating the glass with poly-L-lysine, gelatin, and fibronectin, but the cells still...

17 July 2025 4,900 1 View

Does anyone have suggestions for improving the attachment of endothelial cells from mesenteric arteries to the bottom of the dish?

After dissociating the endothelial cells, I leave them in a calcium-free solution for 2–3 hours. However, I've been having trouble, as only a small percentage of the cells attach to the bottom of...

04 March 2025 704 0 View