Blunt cloning gives no colonies on ligation plate but plenty on control plates. Does anyone have any idea of what's going on?

For the last 2 weeks I have been trying to clone a PCR product into a vector digested with blunt PmeI. I digest the vector for about 6-8 hours, then add CIP to prevent religation. Next, vector is...

03 March 2013 5,380 11 View