Robert Bronstein

3 Questions 4 Answers 0 Followers

Questions related from Robert Bronstein

Is pGL4 luciferase signal purely episomal in nature, is positive hit susceptible to CRISPRi bloackade?

If in a mammalian cell line transfection with a pGL4 luciferase vector harboring some potential cis-regulatory sequence showed a positive hit (e.g. Luciferase expression) then would the same cell...

01 October 2017 1,361 0 View

RNase-A treatment after DNA ethanol precipitation for qPCR

Hi, Wondering about a good protocol for some ChIP eluted DNA, wanted to treat it with RNase A prior to doing some qPCR? Thanks! Rob.

16 May 2017 9,117 1 View

SDS-PAGE w/ PFA fixed tissue?

I am trying to run an SDS-PAGE gel 12% for p53 and alpha-actin and running into some trouble. I am using tissue samples that were fixed with PFA and this is likely the cause of the issues I'm...

22 June 2012 3,380 3 View