Rizwana Ali

4 Questions 4 Answers 0 Followers

Questions related from Rizwana Ali

What is the best fixative for mice adrenal glands either 4% PFA or 10% NBF?

Anyone working on adrenal gland histology, could you please please the protocol to prepare paraffin-embedded mouse adrenals sections? Thank you

10 October 2019 4,693 4 View

If protein samples boiled once for western but store in -20 does it required to boil again although it already denatured?-freeing effect denaturation?

If I boiled the protein samples for western blotting (After BCA and mixing with laemli and beta mercaptoethanol) at 95 degree for 5 minutes and store in -20 to continue western blotting later does...

08 August 2019 7,331 4 View

Mutagenesis Primers calculation: How I can prepare 100 ng/ul of primer working stock?

Can anyone please help me to prepare primer working solution 100ng/ul? As I need 125ng primer concentration for my mutagenesis experiment. I have 100uM stock (Made by diluting 395.9ug in 310.1 ul...

03 March 2019 4,956 5 View

Can anyone please provide the detailed protocol for skeletal muscles isolation for protein extraction as well as BCA assay?

As a beginner I need detailed protocol for protein isolation from specific skeletal muscles of mice such as TA, GAS etc, (for western blot analysis) especially BCA assay I never done before, how...

11 November 2018 5,929 3 View