Riza Arief Putranto

2 Questions 12 Answers 0 Followers

Questions related from Riza Arief Putranto

Does anyone understand what happens to the rest of the all-in-one plasmid carrying sgRNA and Cas9 such as selection antibiotics, etc in the cell?

I am curious about the discussion of organism edited by CRISPR/Cas9 for being considered as non-transgenic material. However, if the plasmid used to carry sgRNA and Cas9 with all antibiotic...

27 November 2015 9,246 4 View

What is the best strategy for designing primer for duplicated genes?

I tried to design primers using several tools but it seems that in reality, I got only several genes after verification by sequencing of the PCR product. I feel like there is some sort of trial...

19 June 2014 9,597 7 View