Riya Philip

5 Questions 4 Answers 0 Followers

Questions related from Riya Philip

Suggest different media for the isolation of bacillus from seaweeds?

Also does anyone use nutrient agar half strength? Do we need to add extra agar for firmness?

14 August 2025 1,540 3 View

Which is better filtered seawater or 1 % saline for serial dilution of sample & media prep for isolation of seaweed associated heterotrophic bacteria?

we use Zobell marine agar media

22 May 2025 412 2 View

Can anyone suggest the composition of Krebs Ringer Phosphate HEPES buffer?

I need this to do glucose uptake assay using 3T3-L1 cells. I checked 2 kit protocols; one by cosmo-bio and other by sigma. Both companies have suggested significantly different buffer composition...

20 January 2016 2,828 5 View

How can I screen natural products/compounds for AMPK activity in 3T3-L1 cells?

I plan to use ELISA kit to measure AMPK activity. This is for screening natural products for potential activators. Should I measure both total and phospho ampk or phospho ampk alone. Can anyone...

15 June 2015 4,463 1 View

Can DAB-H2O2 be used instead of TMB for ELISA?

Infact I plan to coat the plate with tissue lysate. Are there other things one needs to look into? Also should BSA be used while diluting secondary antibody in TBST?

18 February 2014 10,015 1 View