Rituparna Chaudhuri

3 Questions 4 Answers 0 Followers

Questions related from Rituparna Chaudhuri

How do I generate a stable line for a protein that is causing apoptosis of cells?

I added a NLS (nuclear localization sequence) to my clone of interest and expressed it in my cells. However, the protein when expressed in the nucleus of the transfected cells is causing the cells...

02 February 2015 7,258 3 View

How to identify if higher band on western blot is a result of a post translational modification or not?

I fractionate my cells into cytoplasmic and nuclear and consistently get a higher band in the nuclear fraction as compared to cytoplasmic when probed with my antibody of interest. How do I...

11 November 2014 1,411 10 View

Is there any consensus NLS sequence that can be cloned into a protein to localize it to the nucleus?

Hi, can anyone suggest a strong nuclear localization sequence (NLS) that can be cloned into a protein of interest to drive the protein into the nucleus?

08 August 2014 1,387 10 View