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Questions related from Rituparna Chaudhuri
I have used PBMCs from patient blood to generate newly reprogrammed iPSC colonies on using Sendai virus, feeder-free method. I obtained colonies with clear boundaries, but cannot see the signature...
3 weeks ago 1,562 2 View
My newly reprogrammed iPSC colonies were growing extremely slowly. I tested it for Mycoplasma and it came back positive. Since these are precious cells reprogrammed from patient blood, I want to...
08 August 2025 3,468 5 View
Hi, I have successfully reprogrammed PBMCs of 20-30 year old individuals into iPSCs before, using the Sendai virus based Cytotune 2.0 kit. However, I am not getting any colonies from PBMCs of...
17 June 2025 950 3 View
I have generated iPSC colonies from peripheral blood, today is Day14 post-transduction and the colonies seem to have acquired nice borders and distinct iPSC morphology (images attached). There is...
17 December 2021 7,110 1 View
I have calculated the copies/ml of my lentiviral particles using qRT-PCR. Now, to calculate MOI, MOI = plaque forming units of virus for infection/number of cells. In this case, can I equate...
11 November 2021 4,195 4 View
Hi, I am new to iPSCs. I have attached some pictures of newly reprogrammed iPSCs, now at passage 3. They look okay to me, with defined boundaries, but they are not perfectly round, instead have...
02 March 2020 451 18 View
I am folowing the Cytotune 2.0 protocol for feeder-free reprogramming of PBMCs. Everything goes well till 4-5 days after transduction by which time the transduced cells begin to form bigger...
05 February 2020 1,761 14 View
I am generating iPSCs from peripheral blood using Sendai Cytotune 2.0 kit. The cells attached to the surface when shifted to Matrigel coating and formed nice aggregates in the transduced wells by...
03 January 2020 5,352 1 View
If I isolate PBMCs from a donor with an ongoing bacterial infection cold and fever, will the bacteria show up in my PBMC culture ? or just the increased number of WBCs will indicate it?
13 December 2019 6,033 3 View
I fractionate my cells into cytoplasmic and nuclear and consistently get a higher band in the nuclear fraction as compared to cytoplasmic when probed with my antibody of interest. How do I...
04 November 2014 2,233 9 View