Rituparna Chaudhuri

10 Questions 17 Answers 0 Followers

Questions related from Rituparna Chaudhuri

My newly reprogrammed iPSC colonies are growing very slowly and do not show individual cells within the colonies. What is the problem?

I have used PBMCs from patient blood to generate newly reprogrammed iPSC colonies on using Sendai virus, feeder-free method. I obtained colonies with clear boundaries, but cannot see the signature...

last month 1,583 2 View

Does anyone know of any efficient methods to get rid of Mycoplasma in iPSC lines?

My newly reprogrammed iPSC colonies were growing extremely slowly. I tested it for Mycoplasma and it came back positive. Since these are precious cells reprogrammed from patient blood, I want to...

08 August 2025 3,475 5 View

Is reprogramming PBMCs of older individuals into iPSCs more difficult? Does anyone have experience in this?

Hi, I have successfully reprogrammed PBMCs of 20-30 year old individuals into iPSCs before, using the Sendai virus based Cytotune 2.0 kit. However, I am not getting any colonies from PBMCs of...

17 June 2025 963 3 View

ReLeSR or manual passaging at passage 0 after iPSC generation?

I have generated iPSC colonies from peripheral blood, today is Day14 post-transduction and the colonies seem to have acquired nice borders and distinct iPSC morphology (images attached). There is...

17 December 2021 7,132 1 View

Can we calculate MOI of lentivirus from copy number/ml quantified using qRT-PCR?

I have calculated the copies/ml of my lentiviral particles using qRT-PCR. Now, to calculate MOI, MOI = plaque forming units of virus for infection/number of cells. In this case, can I equate...

11 November 2021 4,211 4 View

Do these iPSC colonies look okay?

Hi, I am new to iPSCs. I have attached some pictures of newly reprogrammed iPSCs, now at passage 3. They look okay to me, with defined boundaries, but they are not perfectly round, instead have...

02 March 2020 459 18 View

Why are my transduced PBMCs not attaching to Matrigel?

I am folowing the Cytotune 2.0 protocol for feeder-free reprogramming of PBMCs. Everything goes well till 4-5 days after transduction by which time the transduced cells begin to form bigger...

05 February 2020 1,774 14 View

Why are the aggregated transduced cells not forming a colony or growing after transfer to Matrigel?

I am generating iPSCs from peripheral blood using Sendai Cytotune 2.0 kit. The cells attached to the surface when shifted to Matrigel coating and formed nice aggregates in the transduced wells by...

03 January 2020 5,358 1 View

PBMC isolation from infected individual?

If I isolate PBMCs from a donor with an ongoing bacterial infection cold and fever, will the bacteria show up in my PBMC culture ? or just the increased number of WBCs will indicate it?

13 December 2019 6,040 3 View

How to identify if higher band on western blot is a result of a post translational modification or not?

I fractionate my cells into cytoplasmic and nuclear and consistently get a higher band in the nuclear fraction as compared to cytoplasmic when probed with my antibody of interest. How do I...

04 November 2014 2,243 9 View