Rituparna Chaudhuri

4 Questions 6 Answers 0 Followers

Questions related from Rituparna Chaudhuri

ReLeSR or manual passaging at passage 0 after iPSC generation?

I have generated iPSC colonies from peripheral blood, today is Day14 post-transduction and the colonies seem to have acquired nice borders and distinct iPSC morphology (images attached). There is...

17 December 2021 7,058 1 View

Can we calculate MOI of lentivirus from copy number/ml quantified using qRT-PCR?

I have calculated the copies/ml of my lentiviral particles using qRT-PCR. Now, to calculate MOI, MOI = plaque forming units of virus for infection/number of cells. In this case, can I equate...

11 November 2021 4,148 4 View

Do these iPSC colonies look okay?

Hi, I am new to iPSCs. I have attached some pictures of newly reprogrammed iPSCs, now at passage 3. They look okay to me, with defined boundaries, but they are not perfectly round, instead have...

02 March 2020 401 18 View

How to identify if higher band on western blot is a result of a post translational modification or not?

I fractionate my cells into cytoplasmic and nuclear and consistently get a higher band in the nuclear fraction as compared to cytoplasmic when probed with my antibody of interest. How do I...

04 November 2014 2,187 9 View