Rituparna Chaudhuri

6 Questions 12 Answers 0 Followers

Questions related from Rituparna Chaudhuri

ReLeSR or manual passaging at passage 0 after iPSC generation?

I have generated iPSC colonies from peripheral blood, today is Day14 post-transduction and the colonies seem to have acquired nice borders and distinct iPSC morphology (images attached). There is...

17 December 2021 7,064 1 View

Can we calculate MOI of lentivirus from copy number/ml quantified using qRT-PCR?

I have calculated the copies/ml of my lentiviral particles using qRT-PCR. Now, to calculate MOI, MOI = plaque forming units of virus for infection/number of cells. In this case, can I equate...

11 November 2021 4,150 4 View

Do these iPSC colonies look okay?

Hi, I am new to iPSCs. I have attached some pictures of newly reprogrammed iPSCs, now at passage 3. They look okay to me, with defined boundaries, but they are not perfectly round, instead have...

02 March 2020 403 18 View

Why are my transduced PBMCs not attaching to Matrigel?

I am folowing the Cytotune 2.0 protocol for feeder-free reprogramming of PBMCs. Everything goes well till 4-5 days after transduction by which time the transduced cells begin to form bigger...

05 February 2020 1,721 14 View

Why are the aggregated transduced cells not forming a colony or growing after transfer to Matrigel?

I am generating iPSCs from peripheral blood using Sendai Cytotune 2.0 kit. The cells attached to the surface when shifted to Matrigel coating and formed nice aggregates in the transduced wells by...

03 January 2020 5,329 1 View

How to identify if higher band on western blot is a result of a post translational modification or not?

I fractionate my cells into cytoplasmic and nuclear and consistently get a higher band in the nuclear fraction as compared to cytoplasmic when probed with my antibody of interest. How do I...

04 November 2014 2,193 9 View