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Questions related from Rita Spathis
We have infected mice with an AAV-EGFP and we were hoping to directly visualize the fluorescent signal in frozen muscle sections without antibody staining. Any suggestions would be greatly...
13 April 2023 4,058 3 View
I was having edge effect issues with DAPI staining of spleen frozen sections. After reading over some of the posts on this topic I decided to switch from Fluouromount-G w/DAPI to adding it in...
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I have been attempting to isolate lymphocytes from mouse lymph nodes (inguinal and axillary) with varying and limited success. I have tried to different methods. 1. Place LNs into ice cold PBS....
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