Riddhi Gadre

2 Questions 3 Answers 0 Followers

Questions related from Riddhi Gadre

Can we re-incubate the western blot membrane for the same protein with same primary and secondary antibody?

Hi. I'm trying to extract IFN-G from transfected CHO cells. I have performed western blots a few times with both transfected and non-transfected CHO cell lysate samples, and I'm not getting...

28 May 2023 1,120 3 View

What are the growth characteristics of mammalian cells? Is the mammalian growth curve same as bacterial growth curve?

I am working recombinant protein production using Chinese Hamster Ovarian cells. Need to understand the growth pattern of mammalian cells and how the metabolism rate affects the recombinant...

29 November 2022 8,729 3 View