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Questions related from Richard A Kahn
A graduate student recently asked me this question and I stumbling to give a good answer. We freeze our purified proteins quickly (liquid nitrogen or dry ice/EtOH) to prevent ice crystals from...
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The problem is that the activity is all in the P1 (pellet after homogenization and centrifugation at 1,000xg). When we apply the re-suspended P1 to a sucrose step gradient all the activity is at...
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