Rhiannon Jamieson-Williams

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Questions related from Rhiannon Jamieson-Williams

How to calculate delta-delta CT, and standard deviation, when using biological replicates?

I have 3 experimental conditions, as well as a control. Each of these 4 conditions has 3 biological replicates. When performing the qPCR, each biological replicate was run in triplicate (technical...

27 March 2018 8,180 6 View

Ligated 1.4kb gBlock into pBluescript II KS+. Colonies and liquid culture grows fine, but gel shows low level of DNA. Sequencing faulty. Help?

I am working on setting up a CRISPR/Cas9 experiment. I have successfully cloned my sgRNAs, but due to the size of my deletion, will need to use a vector template instead of an ultramer. The...

31 May 2016 5,694 3 View