Rhea Marie Derke

3 Questions 3 Answers 0 Followers

Questions related from Rhea Marie Derke

Is there a way to add output information to the results of a BLAST search?

I have a list of ~800 protein IDs that I would like to equate to gene names in a different organism (because there is very little annotation for the genome I am working with). I noticed that...

09 November 2020 8,422 3 View

How do I analyze fluorescence polarization (FP) data if FP decreases with protein/DNA binding?

We have generated very consistent FP data that shows decreasing FP with DNA/protein interactions in stead of increasing (as it should be according to the literature). Can anyone help with how I...

26 February 2020 3,818 4 View

Has anyone ever had an E. coli pellet be a bright blue/aqua color after protein expression in ZYP-5052?

I was expressing in BL21(DE3). This is not a reporter protein. There are several metals added to ZYP-5052. After harvesting 2L of cells, lysis, and centrifugation the pellet looked like the...

15 June 2016 9,891 6 View