Rey Sanjorjo

2 Questions 4 Answers 0 Followers

Questions related from Rey Sanjorjo

Is there any sequencing technique where you can sequence DNA with the same size but different sequences?

When we amplify 16S rDNA for sequencing using degenerate primers the amplified gene will result to amplicons with different sequences even with the same DNA band size. I assume that it will be...

28 March 2019 2,811 3 View

What instances that cause false positive colonies during transformation using a purified plasmid?

I transformed a purified 11Kb plasmid (via plasmid mini prep) from an E.coli recombinant strain into the target host via electroporation. I got colonies but there was no DNA band during colony...

17 October 2017 3,777 11 View